- Protein Name
- Mitotic checkpoint serine/threonine-protein kinase BUB1
- Gene Name
- BUB1
- Organism
-
Homo sapiens
- Function
- Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.
| Site |
Maximum Consensus Site Population |
Minimum Consensus Site Population |
Median Consensus Site Population |
| MPP |
10 |
7 |
9.0 |
| DFG |
22 |
22 |
22.0 |
| DRS |
17 |
5 |
14.5 |
| MT3 |
21 |
20 |
21.0 |
| AAS |
21 |
5 |
16.0 |
| ATP |
22 |
3 |
19.5 |
| PDB ID |
Maximum Consensus Site Population |
| MPP site |
DFG site |
PIF site |
DRS site |
DEF site |
EDI site |
MT3 site |
AAS site |
LBP site |
CMP site |
PDIG site |
ATP site |
PMP site |
| Total Druggable Structures per Site |
0 |
1 |
0 |
1 |
0 |
0 |
3 |
3 |
0 |
0 |
0 |
3 |
0 |
|
4R8Q
|
7 |
0 |
0 |
14 |
0 |
0 |
20 |
16 |
0 |
0 |
0 |
21 |
0 |
|
5DMZ
|
9 |
0 |
0 |
17 |
0 |
0 |
21 |
21 |
0 |
0 |
0 |
18 |
0 |
|
AFO43683
|
0 |
22 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
4QPM
|
10 |
0 |
0 |
15 |
0 |
0 |
21 |
17 |
0 |
0 |
0 |
22 |
0 |