- Function
- Together with closely related KSS1, FUS3 is the final kinase in the signal transduction cascade regulating activation/repression of the mating and filamentation pathways, induced by pheromone and nitrogen/carbon limitation, respectively. Phosphorylated FUS3 activates the mating but suppresses the filamentation pathway, whereas activated KSS1 activates both pathways. Pheromone-activated FUS3 functions by inhibiting the binding of the transcriptional activator STE12 to filamentation specific genes while inducing its binding to and activity at mating specific genes. Non-activated FUS3 has a repressive effect on STE12 transcriptional activity. KSS1 can partially compensate for the lack of FUS3 but mating efficiency is reduced and the filamentation program is partially activated upon pheromone signaling. FUS3 phosphorylates STE7, STE5, FAR1, DIG1, DIG2 and STE12.
| Site |
Maximum Consensus Site Population |
Minimum Consensus Site Population |
Median Consensus Site Population |
| MPP |
17 |
10 |
14.5 |
| DFG |
10 |
1 |
6.5 |
| EDI |
23 |
2 |
18.0 |
| MT3 |
18 |
17 |
17.0 |
| AAS |
16 |
4 |
10.5 |
| CMP |
14 |
4 |
8.0 |
| ATP |
13 |
3 |
6.0 |
| PDB ID |
Maximum Consensus Site Population |
| MPP site |
DFG site |
PIF site |
DRS site |
DEF site |
EDI site |
MT3 site |
AAS site |
LBP site |
CMP site |
PDIG site |
ATP site |
PMP site |
| Total Druggable Structures per Site |
2 |
0 |
0 |
0 |
0 |
6 |
2 |
1 |
0 |
0 |
0 |
0 |
0 |
|
2FA2
|
17 |
0 |
0 |
0 |
0 |
16 |
17 |
16 |
0 |
14 |
0 |
9 |
0 |
|
2F9G
|
16 |
5 |
0 |
0 |
0 |
22 |
0 |
0 |
0 |
0 |
0 |
13 |
0 |
|
AFP16892
|
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
2B9I
|
15 |
7 |
0 |
0 |
0 |
21 |
0 |
0 |
0 |
0 |
0 |
12 |
0 |
|
2F49
|
14 |
0 |
0 |
0 |
0 |
23 |
18 |
14 |
0 |
10 |
0 |
6 |
0 |
|
2B9H
|
0 |
10 |
0 |
0 |
0 |
23 |
0 |
0 |
0 |
4 |
0 |
6 |
0 |
|
2B9F
|
15 |
6 |
0 |
0 |
0 |
21 |
0 |
0 |
0 |
0 |
0 |
11 |
0 |
|
2B9J
|
14 |
8 |
0 |
0 |
0 |
14 |
0 |
5 |
0 |
5 |
0 |
13 |
0 |