- Protein Name
- LIM domain kinase 1
- Gene Name
- LIMK1
- Organism
-
Homo sapiens
- Function
- Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).
| Site |
Maximum Consensus Site Population |
Minimum Consensus Site Population |
Median Consensus Site Population |
| DFG |
19 |
3 |
7.0 |
| PIF |
22 |
2 |
17.0 |
| EDI |
22 |
17 |
18.0 |
| MT3 |
25 |
25 |
25.0 |
| AAS |
18 |
16 |
16.5 |
| PDIG |
2 |
2 |
2.0 |
| ATP |
32 |
11 |
22.0 |
| PDB ID |
Maximum Consensus Site Population |
| MPP site |
DFG site |
PIF site |
DRS site |
DEF site |
EDI site |
MT3 site |
AAS site |
LBP site |
CMP site |
PDIG site |
ATP site |
PMP site |
| Total Druggable Structures per Site |
0 |
1 |
4 |
0 |
0 |
4 |
1 |
3 |
0 |
0 |
0 |
2 |
0 |
|
5L6W
|
0 |
7 |
19 |
0 |
0 |
20 |
25 |
0 |
0 |
0 |
0 |
13 |
0 |
|
3S95
|
0 |
9 |
17 |
0 |
0 |
22 |
0 |
17 |
0 |
0 |
0 |
32 |
0 |
|
5HVJ
|
0 |
5 |
22 |
0 |
0 |
17 |
0 |
16 |
0 |
0 |
0 |
22 |
0 |
|
5HVK
|
0 |
0 |
16 |
0 |
0 |
17 |
0 |
18 |
0 |
0 |
2 |
11 |
0 |
|
AFP53667
|
0 |
19 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |