- Protein Name
- AP2-associated protein kinase 1
- Gene Name
- AAK1
- Organism
-
Homo sapiens
- Function
- Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.
| Site |
Maximum Consensus Site Population |
Minimum Consensus Site Population |
Median Consensus Site Population |
| AAS |
20 |
20 |
20.0 |
| MT3 |
5 |
2 |
3.5 |
| LBP |
3 |
3 |
3.0 |
| ATP |
25 |
19 |
22.0 |
| DFG |
20 |
4 |
5.0 |
| DRS |
12 |
12 |
12.0 |
| PDB ID |
Maximum Consensus Site Population |
| MPP site |
AAS site |
MT3 site |
LBP site |
ATP site |
DEF site |
DFG site |
CMP site |
PMP site |
EDI site |
DRS site |
PDIG site |
PIF site |
| Total Druggable Structures per Site |
0 |
1 |
0 |
0 |
2 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
|
AFQ2M2I8
|
0 |
0 |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
0 |
0 |
0 |
0 |
|
4WSQ
|
0 |
0 |
5 |
3 |
25 |
0 |
4 |
0 |
0 |
0 |
12 |
0 |
0 |
|
5L4Q
|
0 |
20 |
2 |
3 |
19 |
0 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |